Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

Table 1 Primer sequences and annealing temperatures (X) used for mRNA analysis

Target	Primer sequence (forward / reverse)	Product size	Annealing temperature [°C]
18S	GCATATGCTTGTCTCAAAGA / CCAAAGGAACCATAACTGAT	101 bp	55 °C
ACE1	AGCCCTCTCAGTGTCTACGC / CTCCTTGGTGATGCTTCCAT	187 bp	57 °C
ADRA1B	TCACGAGGACACCCTTAGCA / GGCTTCAGGGTGGAGAACAA	195 bp	57 °C
ADRA1D	TTCTTCTTTGTCCTGCCGCT / GAAGGCGCGCTTGAACTC	150 bp	57 °C
ADRB2	TGCTGACCAAGAATAAGGCCC / AATGGCATAGGCTTGGTTCGT	173 bp	61 °C
ATIP1	AAGCATTCGTCCAGCAGC / AGAGGTTTCATGCGCAGC	173 bp	55 °C
ATRAP	GAGCTCCTGGTCCACACTG / TAGAACGACCTCCCAGGCA	196 bp	65 °C
EDNRA	AACGAGATGGACAAGAACCGATGT / GACCGAGGTCATCAGACTTTTGGA	195 bp	62 °C
EDNRB	TGCTTGCTTCATCCCGTTCA / ACTTCCCGTCTCTGCTTTAGG	200 bp	59 °C

Cycling conditions: 95 °C, 3 min (one cycle) / 95 °C, 30 sec + X °C, 20 sec + 72 °C, 20 sec (45 cycles) / 72 °C, 5 min (one cycle) / 95 °C, 2 min (one cycle)

ISSN: 1480-9222