Fig. 3

Abundance indicated by qPCR and ddPCR. Ct values measured by qPCR were plotted against the corresponding copies per µl measured for the identical samples by ddPCR. The resulting negative-exponential relationship is highlighted by a trend line. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each