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Fig. 2 | Biological Procedures Online

Fig. 2

From: Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

Fig. 2

Effect size indicated by qPCR and ddPCR. Shown are the ratios of gene expression between stimulated and unstimulated cells, measured by qPCR or ddPCR. The value of 1, which corresponds to a lack of stimulation-induced gene expression change, is highlighted by a vertical dotted line. The measured values are shown as mean values ± standard deviation. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each. ACE1 = angiotensin converting enzyme 1, ADRA1B = alpha-adrenergic receptor type 1B, ADRA1D = alpha-adrenergic receptor type 1D, ATIP1 = angiotensin II receptor interacting protein 1, ATRAP = angiotensin II type 1 receptor associated protein, EDNRA = endothelin receptor type A, EDNRB = endothelin receptor type B

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