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Problem
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Possible reason
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Solution
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The centrifugal unit spun all the protein sample. No sample left on the membrane.
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Faulty filter
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Collect the filtrate and place it onto a new tested and conditioned spin filter
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Filter membrane displaced due to the tight insertion of the filter into the collection tube
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Find a collection tube where a new tested and conditioned filter can be inserted with no friction. Collect the filtrate onto it.
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Low peptide recovery in the filtrate (<40 %)
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Severe filter clogging
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Pipette gently up and down to homogenize when adding a new solution, without touching the filter membrane
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