Table 1 Oligomers used for aTL assay in human and rodent.
From: A quantitative PCR method for measuring absolute telomere length
| Oligomer Name | Species | Oligomer sequence (5'-3') | Amplicon size | |
|---|---|---|---|---|
| Standards | Telomere standard | Human/rodent | (TTAGGG)14 | 84 bp |
| 36B4 standard | Human | CAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGACAAGGCCAGGACTCGTTTGTACCCGTTGATGATAGAATGGG | 75 bp | |
| PCR Primers | teloF | Human/rodent | CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGG TTTGGGTT | >76 bp |
| teloR | Human/rodent | GGCTTGCCTTACCCTTACCCTTACCC TTACCCTTACCCT | ||
| 36B4F | Human | CAGCAAGTGGGAAGGTGTAATCC | 75 bp | |
| 36B4R | Human | CCCATTCTATCATCAACGGGTACAA | ||
| b-globinF | Human | GCTTCTGACACAACTGTGTTCACTAGC | 82 bp | |
| b-globinR | Human | CACCAACTTCATCCACGTTCACC | ||
| 36B4F | Rodent | ACTGGTCTAGGACCCGAGAAG | 78 bp | |
| 36B4R | Rodent | TCAATGGTGCCTCTGGAGATT |